https://docs.google.com/document/d/1NdN9KnTBBm3RF4CpwQ9SSn9buCfDnaZBn94tvILFv5Y/edit?usp=sharing
Names: Bryant Heng, Ezra Ho, Ethan Hong
Class: S2-07
Group Reference: J
Type of research:
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X
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[1] Test a hypothesis: Hypothesis-driven research
e.g. Investigation of the anti-bacteria effect of chrysanthemum
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[2] Measure a value: Experimental research (I)
e.g. Determination of the mass of Jupiter using planetary photography
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[3] Measure a function or relationship: Experimental research (II)
e.g. Investigation of the effect of temperature on the growth of crystals
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[4] Construct a model: Theoretical sciences and applied mathematics
e.g. Modeling of the cooling curve of naphthalene
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[5] Observational and exploratory research
e.g. Investigation of the soil quality in SST
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Category of research:
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Sub-category:
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MICROBIOLOGY
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Bacterial Microbiology
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Reference
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Research Plan
Project Title: Investigation of the effect of UV exposure on the growth of bacteria in the water
1. RATIONALE: Include a brief synopsis of the background that supports your research problem and explain why this research is important and if applicable, explain any societal impact of your research. Include at least 3 in-text citation in your rationale. (500 words)
zΩ
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This research is vital as if our hypothesis is correct, then water filters can screen the bacteria in UV rays many times to kill all bacteria in the water
(Filter Butler, 2018).This would mean that water filters do not need to waste resources like other antimicrobials to ensure that all bacteria are gone
(VictoriaStateGovernment, 2018) and because it can just put the water through many cycles of filtering using the same amount of UV rays, it does not need to be big to output enough UV rays to kill off the bacteria in a one round. (WIKIPEDIA, 2017) Though it may take longer for the water to be filtered, it would cost less and use fewer resources. In the future, water filters may use this as a way to reduce their size and take up less space in homes.
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2. RESEARCH QUESTION(S):
2.1 Research question being addressed
If UV exposure affects the growth of bacteria in the water
2.2 Hypothesis
as the number of times the water is filtered through the filter increases, the lesser the number of bacteria would be in the water
2.2.1 Independent variable
the number of times the bacteria-filled water is filtered through the filter.
2.2.2 Dependent variable
amount of bacteria colonies in the water after the treatment
2.2.3 Controlled variables
1. Amount of water per petri dish
2. The location which the water is obtained from.
3. The initial temperature of the water
2.3 How is this based on the rationale described above?
3. Method
3.1 Equipment list:
- Petri dish x12
- UV water filter
- beaker x3
- Sterilizer
- Water pump
- Pail
Figure 1: Experimental setup
3.3 Procedures: Detail all procedures and experimental design to be used for data collection
1. Pump out water from the school pond enough to fill up 3 Petri dishes.
2. Sterilize the Petri dishes.
3. Fill up the Petri dishes with the same amount of water.
4. Put one Petri dish in the machine
5. Turn the machine on.
6. Take out the Petri dish when done
7. Repeat steps 4 to 6 for L of times
L = Depending on the setup (controlled setup don't filter at all)
3.4 Data Analysis: Describe the procedures you will use to analyze the data/results.
- Mix the sample into a liquid medium, take a small amount of that mixture and further dilute it.
- Repeat this process six to 10 times.
- Spread the final dilution on an agar plate and incubate it for four to seven days before counting the colonies.
- Set the Petri dish on a grid background and count the colonies in each grid cell, moving in a methodical pattern through all of the cells
- NOTE: Plates with colonies that are too numerous to count or with too few colonies need to be re-plated from a new dilution.
4. Risk, Assessment and Management: Identify any potential risks and safety precautions to be taken.
Table 1: Risk Assessment and Management table
Risk
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Assessment
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Management
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The bacteria might infect us and make us sick.
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High
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Students must wear gloves when carrying out the experiment. Students must not eat or drink during the experiment. Students must wash their hands before and after experimentation.
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The machine might fall on our feet and injure our feet
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low
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use a cart to move the machine around and ensure the machine is secure on the cart
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As the experiment involves glassware, there is a risk of breakage and cutting of the hands.
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High
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Wear gloves when carrying out the experiment. In case of breakage, the cut to the hands will be minimized.
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Possibility to trip and fall into the school pond when pumping out the water
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Medium
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Be cautious and don’t go near the edge of the pond.
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5. References: List at least three (3) major sources (e.g. science journal articles, books, internet sites) from your literature review. Choose the APA format and use it consistently to reference the literature used in the research plan. List your entries in alphabetical order for each type of source.
Author last name, first initial. (Publication date). “Article title.” Website title. Retrieved date, from URL
Microbiology Society. “Observing Bacteria in a Petri Dish”. Microbiology Online. Retrieved January 23, 2019, from: https://microbiologyonline.org/teachers/observing-microbes/observing-bacteria-in-a-petri-dish
wikiHow. “How to Filter Water”. wikihow. Retrieved January 24, 2019, from: https://www.wikihow.com/Filter-Water
Becker, A. (March 10, 2018). “How to Count Colonies in Microbiology”. SCIENCING. Retrieved January 24, 2019, from:
6. Bibliography: List at least three (3) major sources (e.g. science journal articles, books, internet sites) from your literature review. Choose the APA format and use it consistently to reference the literature used in the research plan. List your entries in alphabetical order for each type of source.
Microbiology Society. “Observing Bacteria in a Petri Dish”. Microbiology Online. Retrieved January 23, 2019, from: https://microbiologyonline.org/teachers/observing-microbes/observing-bacteria-in-a-petri-dish
wikiHow. “How to Filter Water”. wikihow. Retrieved January 24, 2019, from:
Becker, A. (March 10, 2018). “How to Count Colonies in Microbiology”. SCIENCING. Retrieved January 24, 2019, from:
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