1. Collect 200 ml of water from the school pond with a beaker.
2.Open the biosafety cabinet window and place the agar plates in.
3. Use the micropipette and squirt 100 microlitres of pond water into the agar plates
4. Using a marker, label one half of the agar plates for the control set up and the other half with the time that the agar plate is spent under UV rays, also writing the group (J).
5. Cover half of each agar plate with a tissue to block off UV light for the control setup which is also labelled by a marker on the cover.
6. Place the 3 agar plates under the UV lights for different durations
7. Close the biosafety cabinet window and turn on the UV light.
8. When the time(in seconds) has ended, turn off the UV light and open the biosafety cabinet window and take out the agar plate that is needed
9. After all three agar plates are exposed, use parafilm tape to seal the agar plates (Ensure that the agar plate has tape all around it).
10. After sealing, take the agar plates and put them in the incubator at around 40 degrees
11. When done incubating, observe the patches of bacteria and put the observations into the table and add remarks.
12. Based off these 3 observations, plan for the next three durations which would be most suitable.
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